Production and purification of an Endo1,4-beta-Xylanase from Humicola grisea var. thermoidea by electroelution
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چکیده
منابع مشابه
Purification and Characterization of a New Xylanase from Humicola Grisea Var. Thermoidea
The thermophilic fungus Humicola grisea var. thermoidea secretes extracellular xylanase when grown on solid and in liquid media containing wheat bran and banana plant residue as substrates, respectively. At 55oC, xylanase from the culture filtrate of H. grisea var. thermoidea grown on banana stalk retained 50% of its activity after 28 h of incubation. A xylanase (X2) was isolated from solid sta...
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Glycoside hydrolase family 7 (GH7) cellobiohydrolases (CBHs) play a key role in biomass recycling in nature. They are typically the most abundant enzymes expressed by potent cellulolytic fungi, and are also responsible for the majority of hydrolytic potential in enzyme cocktails for industrial processing of plant biomass. The thermostability of the enzyme is an important parameter for industria...
متن کاملIdentification of an endo-beta-1,4-D-xylanase from Magnaporthe grisea by gene knockout analysis, purification, and heterologous expression.
Magnaporthe grisea, a destructive ascomycetous pathogen of rice, secretes cell wall-degrading enzymes into a culture medium containing purified rice cell walls as the sole carbon source. From M. grisea grown under the culture conditions described here, we have identified an expressed sequenced tag, XYL-6, a gene that is also expressed in M. grisea-infected rice leaves 24 h postinoculation with ...
متن کاملTaxonomy of Scytalidium thermophilum, an important thermophilic fungus in mushroom compost
More than thirty isolates of the thermophilic Torula-Humicola complex, mainly obtained from mushroom compost. were examined and compared with the available type cultures of Torula thermophila, Humicola grisea var. thermoidea and H. insolens. Scytalidium thermophilum is considered to be the correct name for all these isolates and type cultures, but the species is very variable in macroscopical a...
متن کاملEnhancement of Xylanase Production by Protoplast Fusion of Trichoderma spp.
Filamentous fungi Trichoderma spp. are efficient hyper-producers of industrially important enzymes. Xylanases are essential hemicellulases having vast ranges of applications in the various industrial sectors. In this study, the protoplast fusion of two T. virens and </span...
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2003
ISSN: 1517-8382
DOI: 10.1590/s1517-83822003000200007